Development a multiplex RT-PCR assay for simultaneous detection of African swine fever virus, classical swine fever virus and atypical porcine pestivirus
نویسندگان
چکیده
منابع مشابه
A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples.
The development and standardisation of a novel, highly sensitive and specific one-step hot start multiplex RT-PCR assay is presented for the simultaneous and differential diagnosis of African swine fever (ASF) and Classical swine fever (CSF). The method uses two primer sets, each one specific for the corresponding virus, amplifying DNA fragments different in length, allowing a gel-based differe...
متن کاملNested Reverse Transcriptase-PCR (NRT-PCR) Assay for detection of Classical Swine Fever Virus
Classical Swine Fever(CSF) or Hog Cholera is one of the most feared and devastating disease of pigs. The disease has become main threat to pig industry in counrties with a dense pig population and is known to cause more deaths in pigs as compared to many other infectious agents. The disease is endemic in many parts of India including Assam. The virus belongs to genus Pestivirus and family Flavi...
متن کاملDevelopment and Validation of a Multiplex, Real-Time RT PCR Assay for the Simultaneous Detection of Classical and African Swine Fever Viruses
A single-step, multiplex, real-time polymerase chain reaction (RT-PCR) was developed for the simultaneous and differential laboratory diagnosis of Classical swine fever virus (CSFV) and African swine fever virus (ASFV) alongside an exogenous internal control RNA (IC-RNA). Combining a single extraction methodology and primer and probe sets for detection of the three target nucleic acids CSFV, AS...
متن کاملAfrican swine fever virus transcription.
African swine fever virus (ASFV), a large, enveloped, icosahedral dsDNA virus, is currently the only known DNA-containing arbovirus and the only recognized member of the family Asfarviridae. Its genome encodes more than 150 open reading frames that are densely distributed, separated by short intergenic regions. ASFV gene expression follows a complex temporal programming. Four classes of mRNAs h...
متن کاملRapid detection of classical swine fever virus by a portable real-time reverse transcriptase PCR assay.
A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR assay for classical swine fever virus (CSFV) was developed and evaluated in experimentally infected swine. The assay detected CSFV, representing different phylogenetic groupings, but did not amplify viral RNA from related pestiviruses. The assay met or exceeded the sensitivity (1 to 100 50% tissue culture infective doses per ml) ...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2021
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2020.114006